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Potato is a crop grown on all inhabited continents of the globe. It is included in the top five crops of the world, used as staple food in several countries, and the number of people daily consuming the potato may surpass one billion. Despite the high quantities of seed potato produced worldwide, there are yield gaps due to challenges such as abiotic stresses, pests, climate change and poor production practices. A region-wide critical analysis of yield declining factors can help formulate management strategies that can improve potato yields. Bridging yield gaps in potato will ultimately ensure the role of this crop in securing current and future food security. Potato Production Worldwide presents information on this global crop from its history, morphology, and taxonomy to the growth and development of the potato crop, including the latest strategies in addressing today's biotic and abiotic challenges. This book identifies the reasons for yield gaps in various potato production regions of the world, as well as presenting the best production practices, pest management strategies and approaches to deal with climate change from the perspective of potato production. Chapters provide important insights into potato production cultures and approaches in the major potato production countries. Potato Production Worldwide will be a valuable resource for researchers, scientists and students seeking a comprehensive view of successful potato production.
Spatio-temporal expression of insecticidal gene (Cry1Ac& Cry2A) in pre existing lines of transgenic cotton was studied. Seasonal decline in expression of genes differed significantly among different cotton lines tested in the field conditions. The leaves of the Bt cotton plants were found to have the highest levels of toxin expression followed by squares, bolls, anthers and petals. Expression of the genes decreased consistently with the age of plants. Toxin expression in fruiting parts was not enough to confer full resistance against bollworms. The reduction in efficacy of transgenic cotton plants late in the season was attributed to reduction in promoter activity. For this purpose, Rubisco small subunit promoter was isolated from Gossypium arboreum that was further cloned upstream of Cry1Ac in expression vector pCAMBIA 1301. A local cotton cultivar was transformed with Cry1Ac driven by rbcS promoter. The same cotton cultivar was also transformed with Cry1Ac gene driven by 35SCaMV promoter to compare the expression pattern of insecticidal gene under two different promoters. The results showed that rbcS is an efficient promoter to drive Bt gene expression in cotton.
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